[A13868] CAS 54827-17-7 | 3,3′,5,5′-Tetramethylbenzidine, 98%

CAS : 54827-17-7
MDL : MFCD00007748
EINECS : 259-364-6

Other name :

4,4′-Diamino-3,3′,5,5′-tetramethylbiphenylTMB

Chemical Properties

Formula : C16H20N2
Formula weight : 240.35
Melting Point : 168-171°
Sensitivity : Moisture Sensitive. Light Sensitive. Store under Nitrogen. Ambient temperatures.
Form : Powder
Solubility : Soluble in ethyl acetate, ethanol, acetone, chloroform, toluene and dimethyl sulfoxide. Insoluble in water.

Applications

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes; particularly useful in ELISA techniques, blue result can be read spectrophotometrically at 370 or 650 nm3,3′,5,5′-Tetramethylbenzidine is used as a clinical reagent for testing blood. It is also a chromogenic substrate which is used in staining procedures in immunohistochemistry. Further, it is used as a visualizing reagent in enzyme-linked immunosorbent assays. In addition to this, it is used as a replacement for carcinogenic compounds such as benzidine and o-phenylenediamine.

Grade Specifications

GHS Hazard and Precautionary Statements

Hazard Statements : H301-H315-H319-H335Toxic if swallowed.Causes skin irritation.Causes serious eye irritation.May cause respiratory irritation.
Precautionary Statements : P261-P301+P310-P305+P351+P338-P302+P352-P405-P501aAvoid breathing dust/fume/gas/mist/vapours/spray.IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician.IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.IF ON SKIN: Wash with plenty of soap and water.Store locked up.Dispose of contents/container in accordance with local/regional/national/international regulations.

Other References :

Beilstein : 2808541