[J65661] Guanidine hydrochloride, Molecular Biology Grade | Alfa
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Brand: Alfa Aesar
| CH6ClN3 | guanidine
Chemical Identifiers
CAS
50-01-1
Molecular Weight (g/mol)
95.53
Synonym
guanidine
SMILES
[H+].[Cl-].NC(N)=N
InChI Key
PJJJBBJSCAKJQF-UHFFFAOYSA-N
Molecular Formula
CH6ClN3
IUPAC Name
hydrogen guanidine chloride
Specifications
Appearance (Color)
White
Assay (unspecified)
Absorbance (260nm, 6M in water): 0.03 max.
Form
Powder
Assay (Titration)
99.5% min.
DNase
None detected
설명
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Guanidine hydrochloride is used for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Solubility
Freely soluble in water
Notes
Protect from heat. Store away from oxidizing agents.
Other References:
Merck Index:144562Beilstein:3591990
GHS Hazard and Precautionary Statements
Hazard Statements:Serious eye damage/eye irritation (category 2), Skin corrosion/irritation (category 2), Acute toxicity (category 4)
Causes serious eye irritation, May form combustible dust concentrations in air, Causes skin irritation, Harmful if swallowed or if inhaled
Risk & Safety
TSCA:YesRTECS Number:MF4300000
경고:RUO – Research Use Only
CAS50-01-1FORMULACH6ClN3EINECS200-002-3MDLMFCD00013026
| Pakage | 100g, 500g |
|---|---|
| brand | Alfa Aesar |
