02160084-CF | CAS 9007-34-5 | Collagen, Type I from calf skin | MPBIO
Key Features and Details
Collagen
| SKU | 02160084-CF |
| Alternate Names | Collagensoluble; Collagen type I |
| Application Notes | Collagen Type I has been used as a scaffold for the growth of stem cells in vitro, in a wide variety of biomaterial engineering studies. Collagen type I may be used in research of Idiopathic pulmonary fibrosis (IPF). Robust expression of collagen-type 1 is one distinctive feature of IPF. Additionally, collagen-type I has been used in studies on the effect of endoplasmic reticulum (ER) stress from IPF on myofibroblastic differentiation of lung fibroblasts. Collagen-type 1 soluble in acidic solution produces three dimensional scaffolding useful in bioengineering and cell culture applications where biomaterials are needed to replace native collagen extracellular matrices. |
| Base Catalog Number | 160084 |
| CAS # | 9007-34-5 |
| EC Number | 232-697-4 |
| Format | lyophilized, Salt-free, Soluble |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Preparation Method | It is prepared according to the method of Gallop and Seifter. In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M Sodium citrate, pH 3.7, and precipitated as fibrils by dialysis against 0.02 M Na2HPO4.It is prepared according to the method of Gallop and Seifter. In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M Sodium citrate, pH 3.7, and precipitated as fibrils by dialysis against 0.02 M Na2HPO4. |
| Source | Calf skin |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
