|
SKU |
02190679-CF |
|
Alternate Names |
Aldehyde:NAD[P]+ oxidoreductase; ALDH |
|
Application Notes |
Aldehyde dehydrogenase oxidizes a number of aliphatic and aromatic aldehydes. Acetyl-GSH is not hydrolyzed. |
|
Base Catalog Number |
190679 |
|
Biochemical Physiological Actions |
Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme’s activity. Rubidium or NH4+ can be substituted for K+, and glutathione for cysteine. Lithium, Na+, and Cs+ inhibit the reaction. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood. |
|
CAS # |
9028-86-8 |
|
EC Number |
232-845-3 |
|
Extinction Coefficient |
9.4 (279 nm)(Lit) |
|
Format |
Freeze dried stabilized powder, Potassium Activated |
|
Personal Protective Equipment |
Eyeshields, Gloves, respirator filter |
|
Source |
Yeast |
|
Specific Activity |
Protein-~5 u/mg, solid-≥1 u/mg solid |
|
Unit Definition |
One unit causes the oxidation of one µ mole of acetaldehyde to acetic acid per minute at pH 8.0 and 25 °C in the presence of potassium ions and thiols. |
|
Usage Statement |
Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
