02193980-CF | CAS 9001-99-4 | Ribonuclease A | MPBIO
Key Features and Details
Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.
| SKU | 02193980-CF |
| Alternate Names | Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase |
| Application Notes | Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. |
| Base Catalog Number | 193980 |
| Biochemical Physiological Actions | Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides. |
| CAS # | 9001-99-4 |
| EC Number | 232-646-6 |
| Extinction Coefficient | E1%280 = ~7.3 |
| Format | Lyophilized powder, Salt-free and protease-free |
| Grade | Molecular Biology Grade |
| Hazard Statements | H317 |
| Isoelectric Point | 9.45 |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| pH | Optimum pH: 7.0 – 7.5 |
| Preparation Method | Chromatographically Purified |
| RTECS Number | RF0760000 |
| Solubility | Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution. |
| Source | Bovine Pancrease |
| Specific Activity | ≥70 Kunitz units/mg |
| Specificity | The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2′;3′-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains. |
| Unit Definition | One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
