0850341 | IgG1 (Kappa), Clone Mopc 21, mouse ascites | MPBIO
Key Features and Details
[MOPC 21] Ascites fluid, collected from Balb/c mice which carry plasmacytomas subcutaneously, are clarified by centrifugation and filtration. It is dialyzed, adjusted to yield at least 5 mg of immunoglobulin, then vialed and lyophilized. Clarified ascites are tested for immunoglobulin by immunoelectrophoresis using antisera specific for the particular type and isotype. Approximately 10% of the total protein is immunoglobulin (including trace levels of the host’s immunoglobulins), 80% is albumin, and 10% is complement and other proteins.
| SKU | 0850341 |
| Antibody Type | Monoclonal Antibody |
| Application Notes | This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control. |
| Base Catalog Number | 50341 |
| Clone Name | MOPC 21 |
| Conjugate | Unconjugated |
| Formulation Details | Lyophilized |
| Host | Mouse |
| Isotype | IgG1 |
| Pack Size | 5 mg |
| Solubility | Soluble in Water |
| Specificity | The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate (See Protocol for mmunoglobulin Precipitation). In addition; the ascites contains the normal threshold of mouse immunoglobulins and ascites proteins |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
