0855226 | HRP IgGgoat Anti-Human IgG Fc | MPBIO
Key Features and Details
Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to human IgG Fc and buffer salts.
| SKU | 0855226 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase-conjugated goat IgG fraction to human IgG Fc is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is also suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab’)2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from inherent antibodies of host animals. |
| Base Catalog Number | 55226 |
| Concentration | Total Protein: 39.0 mg/mL; Titer: 1:200;000 |
| Conjugate | HRP |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Immunogen | Human IgG (Fc) fragment |
| Isotype | IgG |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. |
| Purity | Purified |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human |
| Typical Working Concentration | ELISA (ELISA) 1:10,000; Immunohistochemistry (Frozen) (IHC (F)) 1:500; Western Blot (WB) 1:1000, 1:500 – 1:4,000 for Tissue Staining; 1:1,000 – 1:8,000 for Immuno Blotting; 1:2,000 – 1:16,000 for ELISA/FIA |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
