0855233 | HRP IgGgoat Anti-Human Kappa Chain (bound) | MPBIO
Key Features and Details
Lyophilized horseradish peroxidase (HRP)-conjugated goat IgG fraction to human Kappa Chain (bound) and buffer salts.
| SKU | 0855233 |
| Alternate Names | Anti-Human |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase-Conjugated Goat IgG Fraction To Human Kappa Chain (Bound) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, cell and tissue labeling (for electron microscopy), and blot immunostaining. |
| Base Catalog Number | 55233 |
| Concentration | Total Protein: 5.8 mg/mL; Titer: 1:181;000 |
| Conjugate | HRP |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Immunogen | Human kappa light chains |
| Isotype | Polyclonal IgG |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Purity | Purified |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Do not add sodium azide. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human |
| Specificity | It shows reactivity to human kappa Fab; cross-reactivity to other species may exist. Because the product is directed against the kappa chains in Fab; some antibodies are expected to bind light chain sites common to all immunoglobulins. |
| Typical Working Concentration | Dilution Recommended: 1:500 – 1:4,000 for Tissue Staining; 1:1,000 – 1:8,000 for Immuno Blotting; 1:2,000 – 1:16,000 for ELISA/FIA |
| Uniprot Number | IGKC_HUMAN (P01834) |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
