0855493 | FITC IgGgoat Anti-Mouse IgG (H&L) | MPBIO
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts.
| SKU | 0855493 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Fluorescein-Conjugated Goat IgG Fraction to Mouse IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed and paraffin-embedded tissue sections. F(ab’)2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. |
| Base Catalog Number | 55493 |
| Concentration | Total Protein: 24.4 mg/mL; Antibody Protein: 10 mg/mL |
| Conjugate | FITC |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Immunogen | Mouse IgG |
| Pack Size | 2 mL |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. |
| Purity | Purified |
| Solubility | Soluble in water |
| Species Reactivity | Mouse |
| Specificity | This product shows reactivity to mouse IgG; cross-reactivity to other species may exist. |
| Typical Working Concentration | Anti-Mouse IgG (whole molecule)-FITC antibody is suitable for use in immunohistochemistry (1:200) and immunofluorescence (1:100). |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
