[0855689] Peroxidase-Conjugated Goat Affinity Purified Antibody to Rabbit IgG (Whole Molecule) – 2mL | MP bio
0855689 | Peroxidase-Conjugated Goat Affinity Purified Antibody to Rabbit IgG (Whole Molecule) – 2mL | MPBIO
Key Features and Details
Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.
| SKU | 0855689 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab’)2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55689 |
| Concentration | Total Protein: 0.8 mg/ml Titer: 1:20;000 |
| Conjugate | HRP |
| Format | lyophilized |
| Formulation Details | Purified |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized. No preservative is added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Rabbit |
| Specificity | It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | SUGGESTED WORKING DILUTIONS: 1:200 – 1:800 for Tissue Staining 1:500 – 1:2,000 for Immunoblotting 1:1,000 – 1:4,000 for Elisa |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
