0855806 | IgG Rabbit Anti-Sheep Red Blood Cells | MPBIO
Key Features and Details
Lyophilized rabbit IgG fraction to sheep red blood cells and buffer salts.
| SKU | 0855806 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers. |
| Base Catalog Number | 55806 |
| Concentration | Total Protein: 20.5 mg/mL Hemagglutination Titer: 1:1600 |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Rabbit |
| Pack Size | 2 mL |
| Preparation Method | The antiserum is prepared by immunizing healthy rabbits with red blood cells obtained from healthy sheep. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material |
| Species Reactivity | Sheep |
| Specificity | The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% sheep red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
