091002417 | Brain Heart Infusion (BHI) broth | MPBIO
Key Features and Details
Brain Heart Infusion Broth is a modification of the media by Rosenow and Hayden. The infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer.
| SKU | 091002417 |
| Alternate Names | BHI Broth; Brain Heart Broth |
| Application Notes | Brain Heart Infusion Broth is developed for the cultivation of a large variety of fastidious and non-fastidious, aerobic and anaerobic microorganisms including bacteria, yeast and fungi (molds). It is very useful for blood cultures. It can also be used to detect organisms in blood of patients on sulfonamide therapy by the addition of PABA (0.05 g/L). |
| Base Catalog Number | 1002417 |
| Format | Solution |
| Formulation Details | Powder | Beef Heart Infusion from Solids 17.5; Dextrose 2.0; Gelatin Peptone 10.0; Disodium Phosphate 2.5; Sodium Chloride 5.0 | Powder |
| Media Type | Yeast Media |
| Pack Size | 500 g |
| pH | 7.2 – 7.6 |
| Preparation Method | Suspend 37 g of the medium in one liter of deionized or distilled water and heat slowly, if necessary. The addition of a small amount of agar (0.1%) is recommended for the growth and isolation of pathogens from blood and other specimen material (anaerobic organisms). Dispense in tubes and sterilize at 121 °C (15lbs. of pressure) for 15 minutes. |
| Solubility | Soluble in 3.7% distilled or deionized water (clear, light to medium amber solution). |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
