Medium 199 with Earle’s Salts (E199), 1g/L Glucose, Glutamine; without Sodium Bicarbonate

Medium 199 with Hanks’ Salts (H199), 1 g/L Glucose, Glutamine; without Sodium Bicarbonate

Ham’s F-12 medium was designed for low density, serum-free growth of Chinese Hamster Ovary (CHO) cells. Ham’s F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells.

RPMI 1640 medium is a general purpose medium based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.