02100617-CF | Elastase, ≥50 U/mg | MPBIO
Key Features and Details
Elastase
| SKU | 02100617-CF |
| Alternate Names | Elastase from hog pancreas; Pancreatopeptidase E |
| Application Notes | Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids. |
| Base Catalog Number | 100617 |
| Biochemical Physiological Actions | Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin. |
| CAS # | 2594837 |
| EC Number | 254-453-6 |
| Format | Solution |
| Formulation Details | Aqueous suspension with 0.01% sodium azide as preservative |
| Grade | 2× Crystallized |
| Hazard Statements | H315-H319-H335 |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| Preparation Method | Elastase is prepared from porcine pancreas. It is lyophilized and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialyzed until chloride-free and then lyophilized. During its preparation the elastase is held below a pH of 5.5 for greater than 24 hours. Two times crystallized from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin. |
| RTECS Number | JX7069000 |
| Safety Symbol | GHS07 |
| Source | Porcine pancreas |
| Specific Activity | Activity ≥50 u/mg protein |
| Unit Definition | One unit will solublize 1 mg of elastin in 20 minutes at 37 °C, pH 8.8. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
