[02100834-CF] CAS 9001-63-2 | Lysozyme, ≥9000 Sugar U/mg solid, from chicken eggwhite | MP bio
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Brand: MP Bio
Lysozyme
02100834-CF | CAS 9001-63-2 | Lysozyme, ≥9000 Sugar U/mg, egg white (MPBIO) | MPBIO
Key Features and Details
Lysozyme
| SKU | 02100834-CF |
| Alternate Names | Muramidase; Mucopeptide N-acetylmuramoylhydrolase |
| Application Notes | Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores. The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Used to prepare spheroplasts. |
| Base Catalog Number | 100834 |
| Biochemical Physiological Actions | Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. |
| CAS # | 9001-63-2 |
| EC Number | 235-747-3 |
| Extinction Coefficient | 26.4 (Lit.) |
| Format | Crystalline powder |
| Hazard Statements | H317 |
| Isoelectric Point | 11.0(Lit.) |
| Molecular Weight | 2235.597 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| RTECS Number | OL5987000 |
| Source | Chicken eggwhite |
| Specific Activity | ≥9000 Sugar U/mg solid |
| Unit Definition | One unit will cause a decrease in OD450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
