[02160047-CF] CAS 53-84-9 | β-Nicotinamide adenine dinucleotide, oxidized form, ≥99% | MP bio
02160047-CF | CAS 53-84-9 | β-NAD⁺, ≥99%, oxidized form, 100mg (MPBIO) | MPBIO
Key Features and Details
β-Nicotinamide Adenine Dinucleotide, Oxidized
| SKU | 02160047-CF |
| Alternate Names | β-DPN; NAD; Nadide; β-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5′-(trihydrogen diphosphate) P’->5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-β-D-ribofuranosylpyridinium hydroxide 5′->5′- ester with adenosine 5′-(trihydrogen pyrophosphate) inner salt |
| Application Notes | Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M. |
| Base Catalog Number | 160047 |
| Biochemical Physiological Actions | Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes. |
| CAS # | 53-84-9 |
| EC Number | 200-184-4 |
| Extinction Coefficient | EmM = 18.0 (260 nm, pH 7)(Lit.) |
| Format | Powder |
| Molecular Formula | C21H27N7O14P2·×H2O |
| Molecular Weight | 663.40 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Purity | ≥99% |
| RTECS Number | UU3450000 |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| UV Visible Absorbance | λ max (water) 259 ± 3 nm |
