02198979-CF | CAS 9032-75-1 | Pectinase | MPBIO
Key Features and Details
Pectinase
| SKU | 02198979-CF |
| Alternate Names | Polygalacturonase; EC 3.2.1.15; Poly-[1,4-alpha-D-galacturonide] glycanohydrolase; Macerozyme R-10 |
| Application Notes | Pectinase is used in plant protoplast preparation to digest cell wall prior to organelle isolation. |
| Base Catalog Number | 198979 |
| Biochemical Physiological Actions | Pectinase causes random hydrolysis of 1,4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. |
| CAS # | 9032-75-1 |
| EC Number | 232-885-6 |
| Format | Powder |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | Asperigillus niger |
| Specific Activity | >1000 u/gm |
| Typical Working Concentration | A concentration of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase), 24 °C to 37 °C for periods of 1 to 16 hours will yield good results for protoplasting. |
| Unit Definition | One unit will liberate one µmole of galacturonic acid from polygalacturonicacid per minute at pH 4.0 at 25 °C. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
