|
SKU |
08320921 |
|
Application Notes |
Zymolyase 20T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration. |
|
Base Catalog Number |
320921 |
|
Formulation Details |
Lyophilized |
|
Pack Size |
1 g |
|
pH |
Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells; pH 6.5, 45 °C for hydrolysis of yeast glucan; pH Range: Stable through pH ranges 5-10 |
|
Protein or Enzyme Type |
Yeast Lytic Enzymes |
|
Source |
Arthrobacter luteus |
|
Specific Activity |
20,000 units/g |
|
Specificity |
The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwannimomyces and others. |
|
Unit Definition |
The amount of activity causing a decrease in absorbance at 800 nm of 30%. |
|
Usage Statement |
Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
