0855168 | FITC IgGgoat Anti-Human Complement C4 | MPBIO
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat IgG fraction to human complement C4 and buffer salts.
SKU | 0855168 |
Antibody Type | Polyclonal Antibody |
Application Notes | FITC-Conjugated Goat IgG Fraction to Anti-Human Complement C4 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. |
Base Catalog Number | 55168 |
Concentration | Total Protein: 7.50 mg/mL |
Conjugate | FITC |
Format | lyophilized |
Formulation Details | Lyophilized |
Grade | Molecular Grade |
Host | Goat |
Pack Size | 2 mL |
Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. |
Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
Species Reactivity | Human |
Specificity | This product shows reactivity to human complement C4; cross-reactivity to other species may exist. Antibody activity to complement components C3; C6-C9 or serum proteins is not present. |
Typical Working Concentration | Dilution reccomendations: 1:100 – 1:400 for Tissue Staining; 1:250 – 1:1,000 for Immuno Blotting; 1:1,000 – 1:4,000 for ELISA/FIA |
Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |