0855500 | FITC IgGgoat Anti-Mouse Complement C3 | MPBIO
Key Features and Details
Lyophilized fluorescein-5-isothiocyanate (FITC “Isomer I”)-conjugated goat IgG fraction to mouse complement C3 and buffer salts.
| SKU | 0855500 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. F(ab’)2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55500 |
| Concentration | Total Protein: 8.3 mg/mL; Antibody Protein: 4 mg/mL |
| Conjugate | FITC |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. |
| Purity | Purified |
| Solubility | Soluble in water |
| Species Reactivity | Mouse |
| Specificity | The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. This product shows reactivity to mouse complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present. |
| Typical Working Concentration | Suggested working dilutions: 1:100 – 1:400 for Tissue Staining; 1:1,000 – 1:4,000 for ELISA |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
