[0855558] Anti-mouse IgG (no cross anti-human) sheep IgG fraction, peroxidase-conjugated, 2 mL | MP bio
0855558 | Anti-mouse IgG (no cross anti-human) sheep IgG fraction, peroxidase-conjugated | MPBIO
Key Features and Details
Lyophilized horseradish peroxidase (HRP)-conjugated sheep IgG fraction to mouse IgG (no cross to human) and buffer salts.
| SKU | 0855558 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase conjugated Sheep IgG fraction to mouse IgG is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab’)2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). It may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. The product can also be used for direct ELISA. |
| Base Catalog Number | 55558 |
| Concentration | Total Protein: 14.9 mg/mL; Titer: 1:187;000 |
| Conjugate | HRP |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Sheep |
| Immunogen | Purified mouse IgG |
| Pack Size | 2 mL |
| Preparation Method | Antibody and highly purified HRP (Rz >3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. |
| Purity | Immunoaffinity Chromatography |
| Solubility | Soluble in water |
| Species Reactivity | Mouse |
| Specificity | Product shows reactivity to mouse IgG. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to human IgG or mouse non-immunoglobulin serum proteins is not present. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
