08771121 | Tubulin, Purified from Bovine Brain | MPBIO
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Key Features and Details
A heterodimer composed of α- and β- tubulin of 55 kDa (molecular weight ≅110 kDa).
| SKU | 08771121 |
| Base Catalog Number | 771121 |
| Format | Liquid |
| Pack Size | 1 mg |
| Preparation Method | One unit of tubulin in G-PEM buffer pH 6.8 plus 10% glycerol was incubated at 35°C in a cuvette. The optical density at 340 nm was taken at time intervals. A value of 1.0 OD unit indicates that >97% of the tubulin has polymerized. The polymerization reaction was performed in duplicate. |
| Purity | 99% (By SDS-PAGE). Single band at 55 kDa. |
| Specificity | When tubulin at a concentration of 1.0 unit/mL (in G-PEM buffer plus 10% glycerol [v/v]) is incubated at 35°C for 30 minutes an increase in optical density (OD340nm) from 0.0 to 1.0 is observed. This corresponds to a total microtubule (MT) polymer mass of >4.8 mg/mL. The mean length of the microtubule population under these conditions is approximately 10 µm as measured by immunoflourescence microscopy and the average number of MTs per ml is 1.0 x 10<sup>12</sup>. |
| Usage Statement | Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
