[08771121] Tubulin, Purified from Bovine Brain | MP bio

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08771121 | Tubulin, Purified from Bovine Brain | MPBIO

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Key Features and Details

A heterodimer composed of α- and β- tubulin of 55 kDa (molecular weight ≅110 kDa).

 

SKU 08771121
Base Catalog Number 771121
Format Liquid
Pack Size 1 mg
Preparation Method One unit of tubulin in G-PEM buffer pH 6.8 plus 10% glycerol was incubated at 35°C in a cuvette. The optical density at 340 nm was taken at time intervals. A value of 1.0 OD unit indicates that >97% of the tubulin has polymerized. The polymerization reaction was performed in duplicate.
Purity 99% (By SDS-PAGE). Single band at 55 kDa.
Specificity When tubulin at a concentration of 1.0 unit/mL (in G-PEM buffer plus 10% glycerol [v/v]) is incubated at 35°C for 30 minutes an increase in optical density (OD340nm) from 0.0 to 1.0 is observed. This corresponds to a total microtubule (MT) polymer mass of >4.8 mg/mL. The mean length of the microtubule population under these conditions is approximately 10 µm as measured by immunoflourescence microscopy and the average number of MTs per ml is 1.0 x 10<sup>12</sup>.
Usage Statement Unless specified otherwise, MP Biomedical’s products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
SKU: 08771121 Category: 
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[08771121] Tubulin, Purified from Bovine Brain | MP bio

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