[32711] CAS 136112-00-0 | Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular | Acros
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Brand: Acros Organics
136112-00-0 | C12H19Cl3O2 | chloroform; isoamyl alcohol; phenol
Chemical Identifiers
CAS
136112-00-0
Molecular Weight (g/mol)
301.63
Synonym
chloroform; isoamyl alcohol; phenol
SMILES
ClC(Cl)Cl.CC(C)CCO.OC1=CC=CC=C1
InChI Key
ZYWFEOZQIUMEGL-UHFFFAOYSA-N
Molecular Formula
C12H19Cl3O2
IUPAC Name
3-methylbutan-1-ol; phenol; trichloromethane
Specifications
Appearance (Color)
Clear yellow to orange
Appearance (Form)
Solution
Heavy metals (as Pb)
=<1 ppm
pH
7.8 to 8.2 (at 25°C)
Iron (Fe)
=<1 ppm
설명
This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
- This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0
Applications
- Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
- When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
- It is also used to generate DNA minisatellite fingerprints
Other References:
Merck Index:14, 7241UN Number:2927
GHS Hazard and Precautionary Statements
Hazard Statements:Germ cell mutagenicity (category 2), Reproductive toxicity (category 2), Specific target organ toxicity after repeated exposure (category 1), Skin corrosion/irritation (category 1), Specific target organ toxicity after single exposure (category 3), Carcinogenicity (category 2), Acute toxicity (category 3)
Suspected of causing genetic defects, Suspected of damaging the unborn child, Causes damage to organs through prolonged or repeated exposure, Causes severe skin burns and eye damage, May cause drowsiness or dizziness, Suspected of causing cancer, Repeated exposure may cause skin dryness or cracking, Toxic if swallowed, in contact with skin or if inhaled
Risk & Safety
경고:RUO – Research Use Only
CAS136112-00-0FORMULAC12H19Cl3O2MDLMFCD00133763PACKAGING GROUPII
| Pakage | 100ml, 2.5L, 500ml |
|---|---|
| brand | Acros Organics |
