[J62216] CAS 868272-85-9 | 3,3′-Diaminobenzidine tetrahydrochloride hydrate, 97% | Alfa

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Brand: Alfa Aesar

868272-85-9 | C12H18Cl4N4 | 3,3′-diaminobenzidine tetrahydrochloride

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Chemical Identifiers

CAS
868272-85-9
Molecular Weight (g/mol)
360.10
MDL Number
MFCD08273058
Synonym
3,3′-diaminobenzidine tetrahydrochloride
SMILES
Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1
InChI Key
KJDSORYAHBAGPP-UHFFFAOYSA-N
Molecular Formula
C12H18Cl4N4
IUPAC Name
[1,1′-biphenyl]-3,3′,4,4′-tetramine tetrahydrochloride

Specifications

Assay (unspecified)
≥97%
Form
Powder or crystalline powder
Appearance (Color)
Pale grey to grey to dark brown or brown-purple
Loss on Drying
≤10.0 % (120°C/constant weight)

설명

3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3′-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• 3,3′-Diaminobenzidine tetrahydrochloride hydrate is an organic compound used as a peroxidase substrate

Application

• 3,3′-Diaminobenzidine tetrahydrochloride hydrate is commonly used for immunoblotting and immunohistochemical staining techniques

• It has also been used for the histological stain to measure the peroxidase activity. The interaction between 3,3′-Diaminobenzidine tetrahydrochloride hydrate and peroxidase leads to the formation of a visible insoluble product. Therefore, it has been applied to measure glutamate decarboxylase activity in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces

• It is used as a chromogen for detecting the antigen-antibody complex

• This compound is also used in several laboratory applications, including electron and light microscope, and FISH (fluorescence in situ hybridization)

GHS Hazard and Precautionary Statements

Hazard Statements:H315-H320-H335-H341-H350
GHS H StatementH351-H315-H319-H335 Suspected of causing cancer.Causes skin irritation.Causes serious eye irritation.May cause respiratory irritation.
Precautionary Statements:P201-P202-P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P308+P313-P332+P313-P362-P501c

Risk & Safety

TSCA:Yes

경고:RUO – Research Use Only

CAS868272-85-9FORMULAC12H18Cl4N4MDLMFCD08273058

Pakage

10g, 5g

brand

Alfa Aesar

SKU: J62216 Category:  Brand:
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[J62216] CAS 868272-85-9 | 3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97% | Alfa

[J62216] CAS 868272-85-9 | 3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97% | Alfa

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